365赌球

王义强; 林方睿; 胡睿; 刘丽炜; 屈军乐

doi:10.37188/CO.2022-0098

365赌球

doi: 10.37188/CO.2022-0098

王义强 ^{1, 2,},
林方睿 ^{1, 2,},
胡睿 ^{1, 2,},
刘丽炜 ^{1, 2,},
屈军乐 ^{1, 2,,}

1.
深圳大学物理与光电工程学院, 广东深圳 518060
2.
光电子器件与系统教育部/广东省重点实验室, 广东深圳 518060

基金项目:国家自然科学基金资助项目（No. 62127819）

详细信息

作者简介:
王义强（1996—），男，安徽合肥人，硕士研究生，2018年于安徽大学光电信息科学与工程专业获得学士学位，主要从事荧光显微成像系统的研究。E-mail：[email protected]

林方睿（1993—），男，福建武夷山人，2015年于兰州理工大学光电信息科学与工程专业获得学士学位，2018年于华南师范大学光学工程专业获得硕士学位，2022年于深圳大学获得博士学位。主要从事荧光显微成像系统的研究。E-mail：[email protected]

胡　睿（1983—），男，博士，副教授。2004年于浙江大学信息工程学院获得学士学位，2010年于浙江大学获得光学工程博士学位。2012—2015年于新加坡南洋理工大学从事博士后研究。主要从事生物医学光子学相关领域的研究工作，近年来专注于纳米颗粒与细胞的相互作用，通过光学成像及光谱学分析等方法研究二者相互作用过程的细节问题。E-mail：[email protected]

刘丽炜（1980—）女，吉林长春人，博士，教授，2003年、2009年于长春理工大学分别获得学士、博士学位。研究方向：生物医学光子学。E-mail：[email protected]

屈军乐（1970—），男，陕西西安人，博士，教授，1992年毕业于西安交通大学电子工程系，获工学学士学位；1995年和1998年在中国科学院西安光学精密机械研究所分别获得理学硕士和博士学位；2001年8月到2003年6月在美国印第安纳大学从事博士后研究；2003年7月回国至今在深圳大学物理与光电工程学院工作。研究方向：生物医学光子学。E-mail：[email protected]

中图分类号:O435.2;O438.2
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365赌球注册开户
- 收稿日期: 2022-05-13
- 录用日期: 2022-07-07
- 修回日期: 2022-05-31
- 网络出版日期: 2022-08-03

365赌球官网平台

WANG Yi-qiang ^{1, 2
,},
LIN Fang-rui ^{1, 2
,},
HU Rui ^{1, 2
,},
LIU Li-wei ^{1, 2
,},
QU Jun-le ^{1, 2
,,}

1.
College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China
2.
Key Laboratory of Optoelectronic Devices and Systems of the Ministry of Education/Guangdong Province, Shenzhen 518060, China

Funds:Supported by National Natural Science Foundation of China (No. 62127819)

More Information

Corresponding author: [email protected]

摘要

摘要:
光学显微成像技术具有实时性、高分辨率和非侵入性等特点，其成像尺度可跨越细胞、组织乃至生命体，极大地拓展了人们对生命本质的认识边界。然而，受限于光学显微成像系统有限的空间带宽积（Space-Bandwidth Product，SBP），常规的光学显微镜难以同时兼具大视场和高分辨率，使得显微成像在大视场生物成像应用中受到较大的限制，例如，对脑神经网络以突触为单位的神经回路成像。近年来，365赌球得到不断的发展，其SBP的视场相较于传统的光学显微镜有了十倍甚至百倍的提升，在保持高分辨率的基础上拓展了成像视场，从而可以满足生物医学领域重大问题的研究需求。本文介绍了近年来几种典型的365赌球及其生物医学应用，并对其未来发展做了展望。
- 光学显微 /
- 空间带宽积 /
- 大视场成像 /
- 活体成像
Abstract:
With the characteristics of real-time, high-resolution and non-invasive, optical microscopy can scale from cells, tissues to whole living organisms, which has greatly expanded our understanding to the nature of life. However, due to the limited Space-Bandwidth Product (SBP), it is hard for a conventional optical microscope to achieve a large field of view with a high resolution. This makes it very difficult for microscopic imaging in large field of view biological imaging applications, such as imaging of neural circuits between the synapse of the brain neural networks. Recently, large field-of-view imaging technology has received increasing attention and experienced rapid development. The SBP has been improved ten times or even a hundred times as compared to a traditional optical microscope and the field-of-view has been expanded without sacrificing resolution, which, in turn, has resolved some major problems in biomedical research. This review introduces the progress, characteristics and corresponding biological applications of several typical trans-scale optical imaging techniques in recent years, and gives an outlook on their future development.
- optical microscopy /
- space-bandwidth product /
- large field-of-view imaging /
- in vivo imaging

HTML全文

图 1 大视场物镜双光子脑成像^{[
12
]}。(a)成像系统光路图和物镜实物图（左上）；(b) 活体鼠脑神经细胞的双光子成像，深度为150 μm；(c)图(b)中虚线框内的细节放大图

Figure 1. Large FOV objective two-photon brain imaging^{[
12
]}. (a) Optical path diagram of the imaging system and objective lens (upper left); (b) two-photon imaging of neuronal cells activity of mice brain in vivo, the depth of imaging is 150 μm; (c) magnification of some details in white box of (b)

下载: 全尺寸图片幻灯片

图 2 基于散斑光片照明的大视场成像系统^{[
25
]}。(a) 系统光路示意图，激光经磨砂玻璃片形成的散斑图案投影在体积为4.4 mm×3 mm×3 mm的样品内，形成厚度约为3 μm的散斑光片照明；(b) 用光片照明模式实现斑马鱼全身成像；(c)用共聚焦模式实现的斑马鱼全身成像

Figure 2. Speckle light sheet illumination-based large FOV imaging system^{[
25
]}. (a) System setting, the speckle pattern formed by the laser through the ground glass disk is projected into the sample with a volume of 4.4 mm×3 mm×3 mm, forming a speckle light sheet with a thickness of about 3 μm for illumination; (b) Zebrafish whole body imaging with light sheet illumination; (c) Zebrafish whole body imaging with confocal microscopy

下载: 全尺寸图片幻灯片

图 3 微透镜的排列与扫描方向示意图^{[
37
]}

Figure 3. Schematic diagram of microlenses arrangement and scanning direction

下载: 全尺寸图片幻灯片

图 4 阵列显微系统^{[

39
]}。 (a) 阵列显微系统光路设置；(b) 小鼠肾脏切片的高通量成像；(c)图(b)中方框内的放大图

Figure 4. Array microscopy system^{[

39
]}. (a) The optical setting of array microscopy; (b) high-throughput imaging of mouse kidney slices; (c) magnification of the rectangle in (b)

下载: 全尺寸图片幻灯片

图 5 无透镜分辨率增强成像系统^{[
21
]}。(a) 系统结构和其使用的无序表面示意图；(b) 系统对小鼠肾脏切片的高通量成像，并选取三个子区域b1, b2, b3（图中红色框内）的成像结果与使用20 X, 0.75 NA物镜的荧光显微镜成像结果做对比，两者的相似度约为0.75

Figure 5. Resolution enhanced lensless imaging system^{[
21
]}. (a) Schematic diagram of system design and the disordered surface; (b) high-throughput imaging of mouse kidney slices. Imaging results of three sub-regions b1, b2, b3 (red boxes) are compared with that of the fluorescence microscope using an objective with 20 X and 0.75 NA. The similarity is about 0.75

下载: 全尺寸图片幻灯片

表 1 4类典型的365赌球参数对比

Table 1. Comparison of four representative optical microscopy imaging techniques with large FOV

技术类别	成像方式	分辨率/μm	帧率（frame·s⁻¹）	视场	优缺点	适用场景
大视场物镜成像	宽场^{[ 27 ]}	0.7	92	20 mm²	可兼容多种成像方式；像质分布不均匀	活体、细胞、切片观察
	双光子^{[ 17 , 27 ]}	0.6	5×10⁻³	20 mm²
	光片^{[ 29 ]}	0.7	0.15	20 mm²

曲面探测成像	串行成像^{[ 37 ]}	1.5	0.7	1256 mm²	整体像质更好；成像方式多局限于宽场	活体、细胞、切片观察
曲面探测成像	并行成像^{[ 19 ]}	1.2	30	113 mm²	整体像质更好；成像方式多局限于宽场	活体、细胞、切片观察

阵列显微	宽场+扫描^{[ 41 ]}	1.7	6	60 mm²	简单；焦深浅	切片观测

无透镜显微	多波长复用^{[ 53 ]}	0.69	0.5	29 mm²	简单，低成本；成像保真度有限	细胞、切片观测
	倾斜成像^{[ 55 ]}	0.69	5.6×10⁻²	120 mm²
	编码叠层成像^{[ 26 ]}	0.3	6.7×10⁻²	240 mm²

下载: 导出CSV

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